id author title date pages extension mime words sentences flesch summary cache txt cord-309107-2xzam3x9 Emmler, Laura Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis 2019-11-15 .txt text/plain 4623 228 54 title: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis This study investigated the presence of FCoV with and without S gene mutations in cats with FIP using two different real-time RT-PCRs on different samples obtained under clinical conditions. METHODS: Fine-needle aspirates (FNAs) and incisional biopsies (IBs) of popliteal and mesenteric lymph nodes, liver, spleen, omentum and kidneys (each n = 20), EDTA blood (n = 13), buffy coat smears (n = 13), serum (n = 11), effusion (n = 14), cerebrospinal fluid (n = 16), aqueous humour (n = 20) and peritoneal lavage (n = 6) were obtained from 20 cats with FIP diagnosed by immunohistochemistry. This study investigated the presence of FCoV with and without S gene mutations in different tissue and body fluid samples from cats with IHC-confirmed FIP via realtime RT-PCR. ./cache/cord-309107-2xzam3x9.txt ./txt/cord-309107-2xzam3x9.txt