id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308422-ueyaw8pd	Wong, Christopher W	Optimization and clinical validation of a pathogen detection microarray	2007-05-28		.txt	text/plain	6487	298	43	Here, we report the results of a systematic investigation of the complex relationships between viral amplification efficiency, hybridization signal output, target-probe annealing specificity, and reproducibility of pathogen detection using a custom designed microarray platform. The array was designed to detect up to 35 RNA viruses using 40-mer probes tiled at an average 8-base resolution across the full length of each genome (53,555 probes; Figure S1 and Table S1 in Additional data file 1). We next hypothesized that amplification efficiency scoring could be used to select an optimal tag sequence (that is, for the RT-PCR primers) for achieving uniformly high AES Table 1 Binning of probes into specific pathogen signature probe sets across viral genomes, thus globally maximizing PCR efficiency (see supplemental methods in Additional data file 1 and [25] ).	./cache/cord-308422-ueyaw8pd.txt	./txt/cord-308422-ueyaw8pd.txt