id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307068-360qs3ov	Hagiwara, Masanori	Loop‐mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18	2007-03-26		.txt	text/plain	3332	194	56	A new method was developed for detection of human papillomavirus (HPV) by loop‐mediated isothermal amplification (LAMP), which was compared with the polymerase chain reaction (PCR), and real‐time PCR for specificity and sensitivity. In order to evaluate the reliability of HPV type‐specific LAMP detecting HPV DNA from clinical samples, tissue specimens were obtained from 27 patients with external genital polypoid lesions. In this study, a LAMP-based HPV typespecific DNA amplification method was developed and were compared its specificity and sensitivity with PCR and real-time PCR. Type-specific real-time PCR was used to measure the quantity of the DNAs of HPV-6, -11, -16, and -18 in each sample. The sensitivity of HPV-6, -11, -16, and -18 type-specific LAMP determined by turbidity assay were 1,000 copies/tube (Fig. 3) . The sensitivity of amplification of LAMP for detection of viral DNA was nearly the same as that of real-time PCR.	./cache/cord-307068-360qs3ov.txt	./txt/cord-307068-360qs3ov.txt