id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305872-66vij492	Caasi, Donna Ria J.	A multi-target, non-infectious and clonable artificial positive control for routine PCR-based assays	2013-09-05		.txt	text/plain	4830	228	47	To test this concept, artificial positive controls (APCs) for use in PCR were synthesized to contain primer sequences targeting four viruses (Barley yellow dwarf virus, Soilborne wheat mosaic virus, Triticum mosaic virus and Wheat streak mosaic virus) pathogenic to wheat and, as internal control, the plant mitochondrial nad5 gene. Plasmid construct-based synthetic controls harboring a custom and de novo designed insert can enhance technologies such as polymerase chain reaction (PCR) to improve biosafety, speed of processing and overall detection without compromising sensitivity and specificity. The Plot ΔG or energy dot plot contains the Table 1 Primers used for designing the APC synthetic inserts, performing PCR of the targets within APCs, and RT-PCR of in vivo reference positive controls a . PCR products from APCs may differ in size from those generated by traditionally-used positive controls (Fig. 2) due to the discrepancy in the lengths between the annealing sites of the target sequences in vivo and the arrangement of primer sequences in the APC template (Table 1) .	./cache/cord-305872-66vij492.txt	./txt/cord-305872-66vij492.txt