id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305336-wxiazglk	Li, Ji Lian	Systemic Spread and Propagation of a Plant-Pathogenic Virus in European Honeybees, Apis mellifera	2014-01-21		.txt	text/plain	6907	319	50	In the present study, we showed that a plant-pathogenic RNA virus, tobacco ringspot virus (TRSV), could replicate and produce virions in honeybees, Apis mellifera, resulting in infections that were found throughout the entire body. While intracellular life cycle, species-level genetic variation, and pathogenesis of the virus in honeybee hosts remain to be determined, the increasing prevalence of TRSV in conjunction with other bee viruses from spring toward winter in infected colonies was associated with gradual decline of host populations and winter colony collapse, suggesting the negative impact of the virus on colony survival. Conventional RT-PCR was performed on RNA samples extracted from adult bees, Varroa mites, different tissues, and bee bread collected from the same colony for the presence and distribution of TRSV.	./cache/cord-305336-wxiazglk.txt	./txt/cord-305336-wxiazglk.txt