id author title date pages extension mime words sentences flesch summary cache txt cord-304913-qb9zeazk Thibivilliers, Sandra Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection 2009-04-27 .txt text/plain 6680 344 52 RESULTS: A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene Ur-4. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation) mainly due to the presence of the Avr4 gene in the race 49 leading to a hypersensitive response in the bean plants. The stability of the expression level of the 3 remaining genes, TC127, cons6 and cons7 was evaluated by qRT-PCR on cDNAs from bean uninfected or infected with bean rust race 41 or 49 at 6, 12, 24, 48, 72, and 96 hours after inoculation (HAI). ./cache/cord-304913-qb9zeazk.txt ./txt/cord-304913-qb9zeazk.txt