id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303818-z3js3mr4	Chen, Huixin	Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses	2015-10-11		.txt	text/plain	3836	196	51	title: Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses In the present study, we aimed to develop and evaluate a SYBR Green Iebased multiplex real-time RT-PCR assay to detect, differentiate, and quantify DENV and CHIKV and simultaneously to serotype DENV based on Tm analysis of the PCR amplicon. A set of 22 serum samples from CHIKV-infected patients and 30 from uninfected individuals were collected at the National University Hospital, Singapore, with informed consent, to evaluate the clinical sensitivity and specificity of the real-time RT-PCR assay. To our knowledge, this is the first SYBR Green Iebased real-time RT-PCR assay reported that is able to detect, quantify, differentiate, and serotype DENV and CHIKV simultaneously. Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection Development of group-and serotype-specific one-step SYBR green I-based real-time reverse transcription-PCR assay for dengue virus	./cache/cord-303818-z3js3mr4.txt	./txt/cord-303818-z3js3mr4.txt