id author title date pages extension mime words sentences flesch summary cache txt cord-301430-gzou8b9k Beier, D. Establishment of a new bovine leukosis virus producing cell line 2004-08-17 .txt text/plain 4086 248 58 In addition, earlier investigations demonstrated that contamination of the BLV antigen-producing cell culture systems by bovine viral diarrhea virus (BVDV) may give rise to misinterpretation of serological test results after BVDV vaccination of cattle. Investigations of a panel of well-characterised sera by agar gel immunodiffusion (AGID) and capture ELISA (cELISA) tests using antigen prepared from this new cell line in comparison with antigen of the well-known cell line FLK/BLV yielded comparable results. Standard and field sera were investigated in parallel with a commercially available test kit (Riemser Rinderleukose Test Kit, RTAM/Germany) in order to compare sensitivity and specificity of the antigens from the cell lines PO714 and FLK/BLV. Comparative investigations of sera from cattle infected with BLV of different provirus subtypes with antigen from both cell lines using the gp51 cELISA. ./cache/cord-301430-gzou8b9k.txt ./txt/cord-301430-gzou8b9k.txt