id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301167-101lnq4f	Liu, Quanjun	Microarray-in-a-Tube for Detection of Multiple Viruses	2007-02-01		.txt	text/plain	3248	177	48	Methods: We developed a novel PCR assay, the microarray-in-a-tube system, which integrates multiple PCR processes and DNA microarrays for multiple virus detection. A 5 × 5 oligonucleotide microarray for detecting 4 respiratory tract viruses (severe acute respiratory syndrome–associated coronavirus, influenza A virus, influenza B virus, and enterovirus) with inner controls was arranged on the inner surface of a specially designed Eppendorf cap with a flat, optically transparent window. We aimed to develop a microarray-in-a-tube that integrates RT-PCR and a DNA microarray for detecting and distinguishing 4 viruses causing human acute respiratory tract infection, SARS coronavirus, influenza A and B viruses, and enterovirus. The system (Fig. 1 ) has 3 parts, which include an optically transparent plastic cap with an oligonucleotide microarray on the inner surface, a black inner vessel that contains hybridization solution, and the body of the Eppendorf tube.	./cache/cord-301167-101lnq4f.txt	./txt/cord-301167-101lnq4f.txt