id author title date pages extension mime words sentences flesch summary cache txt cord-300685-bcjnujlj Poon, Leo L M Rapid Diagnosis of a Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS) 2003-06-01 .txt text/plain 2425 115 54 The detection of live virus (4 ) and the detection of high copy numbers of viral sequence from NPA samples in the current study clearly support that the concept that cough and sneeze droplets from SARS patients are a major route of spread of this infectious agent. Interestingly, two of four available stool samples from the SARS patients in this study were positive in the assay (data not shown). RNA samples from this study were subjected to nested reverse transcription-PCR (4 ), and no evidence of metapneumovirus infection was detected in any of the patients in this study (data not shown), suggesting that the novel coronavirus is the key player in the pathogenesis of SARS. The PCR products from all 23 positive cases in this study had the same melting point, strongly suggesting that there was no viral sequence variation in the target region of samples collected at the two Hong Kong hospitals during the 1-month period of patient accrual. ./cache/cord-300685-bcjnujlj.txt ./txt/cord-300685-bcjnujlj.txt