id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299537-lbx1plqx	Wang, Wei	Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai	2010-09-19		.txt	text/plain	3998	227	53	METHODS: A 2-year surveillance program of children presenting with acute respiratory infections (ARIs) was carried out to characterize the viral etiology and to assess whether using gene amplification and sequencing could be a reliable approach to monitor virus introduction and spread in a population subgroup. RESULTS: Using multiplex RT-PCR, 15 different respiratory viruses were detected within the 486 nasopharyngeal positive samples collected among 817 children aged <9-year old who presented with ARI during October 2006 to September 2008. CONCLUSIONS: This study supports the usefulness of multiplex RT-PCR for virus detection and co-infection, and for implementation of a molecular monitoring system for endemic and epidemic viral respiratory infections. In the present 2-year study, we used a five-tube mRT-PCR assay we implemented in the Pasteur Institute network in the Asian region (http://www.pasteur-international.org/ip/easysite/ pasteur-international/activites-scientifiques/projets/tous-lesprojets/sisea), which covered 17 common respiratory viruses, 10 to identify viruses in nasopharyngeal specimens in 817 children with Table 1 Criteria of patient enrollment.	./cache/cord-299537-lbx1plqx.txt	./txt/cord-299537-lbx1plqx.txt