id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295401-3p6q92x4	Gueudin, M	Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay	2003-02-18		.txt	text/plain	3975	206	60	title: Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay RSV and GAPDH were quantified in nasal aspirates from 75 children hospitalised for acute respiratory tract disease: 31 (41.3%) were positive according to the immunofluorescence assay (IFA), 34 (45.3%) were culture-positive and 42 (56%) were positive according to our real-time RT-PCR method. RNA and DNA extracted from cell cultures infected with different respiratory viruses were screened by the real-time RT-PCR for RSV to assess the specificity of this assay. Of the 42 samples found to be positive by the real-time RT-PCR assay for RSV, six could not be quantitated as the number of RSV RNA copies contained was below the detection limit of the technique; two of these six samples were also culturepositive. The sensitivity of the real-time RT-PCR assay for RSV on clinical samples was 56%, which is significantly higher than the sensitivity of conventional techniques for the detection of RSV in nasal aspirates.	./cache/cord-295401-3p6q92x4.txt	./txt/cord-295401-3p6q92x4.txt