id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295296-jtjx1vgd	Tiwari, Shashi Kant	`In-silico primer designing and PCR for detection of novel coronavirus-19	2020-10-23		.txt	text/plain	569	43	67	title: 'In-silico primer designing and PCR for detection of novel coronavirus-19 Novel corona virus (2019-nCoV or COVID-19) pandemic has been considered as a major public health emergency in the world of this century. Novel corona virus (2019-nCoV or COVID-19) pandemic has been considered as a major public health emergency in the world of this century. qRT-PCR primer and probe were designed within conserved region from whole genome, RNA dependent RNA polymerase (RdRP) and envelope to detect SARS-CoV2 from COVID-19 virus isolated from Wuhan, China (Gene bank No: NC_045512, MT072668.1and MT111896.1 respectively). All these sets of primers of COVID-19 were highly specific and any of the primer set either syber green based or Taqman probe based may be useful for commercial one step RT-PCR based kit development for the molecular diagnosis of COVID-19 viruses. The assay based on one step qRT-PCR detection will be sensitive, rapid and specific to detect COVID-19 viruses in mouth swab, nasal swab, 	./cache/cord-295296-jtjx1vgd.txt	./txt/cord-295296-jtjx1vgd.txt