id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293590-0xn6mqh6	Peña, Andrea A	An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV	2010-04-14		.txt	text/plain	3724	187	48	FINDINGS: The expression stability of five commonly used housekeeping genes [beta-actin (ACTB), elongation factor 1-alpha (EF1A), ubiquitin (UBQ), glyceraldehyd-3-phosphate dehydrogenase (GAPDH) and tubulin alpha (TUBA)] were monitored in salmonid cell lines CHSE-214 and RTS11 after infection with two of the most fastidious fish pathogens, the facultative bacterium Piscirickettsia salmonis and the aquabirnavirus IPNV (Infectious Pancreatic Necrosis Virus). CONCLUSION: Based on the data presented here with the cell culture models CHSE-214 and RTS11, we suggest the initial choice of UBQ, ACTB and EF1A as reference genes in qRT-PCR assays for studying the effect of P. Prior validation of the selected reference gene candidates (ACTB, EF1A, GAPDH, UBQ and TUBA), general expression levels based on mean qPCR threshold cycle (Ct) values in control CHSE-214 and RTS11 cells were determined, since extremely high or low expression levels might preclude their usefulness as internal controls ( Figure 3) .	./cache/cord-293590-0xn6mqh6.txt	./txt/cord-293590-0xn6mqh6.txt