id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292031-weiwksh6	Ramírez-Castillo, Flor Yazmín	Waterborne Pathogens: Detection Methods and Challenges	2015-05-21		.txt	text/plain	7358	378	36	Quantitative microbial risk assessment (QMRA) is a helpful tool to evaluate the scenarios for pathogen contamination that involve surveillance, detection methods, analysis and decision-making. Molecular techniques, such as nucleic acid amplification procedures, offer sensitive and analytical tools for detecting a variety of pathogens, including new emerging strains, present the possibility of automation, and real-time analysis to provide information for microbial risk assessment purposes [33] . Limitations of DNA based methods such as PCR include the inability to discriminate between viable from non-viable cells that both contain DNA, the low concentration of several pathogens in water such as Cryptosporidium, Giardia and viruses, and the lack of data to indicate the real infectious risk to a population. Oligonucleotide microarrays are a powerful genomic technology that is widely utilized to monitor gene expression under different cell growth conditions, detecting specific mutations in DNA sequences and characterizing microorganisms in environmental samples [76] .	./cache/cord-292031-weiwksh6.txt	./txt/cord-292031-weiwksh6.txt