id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291958-g4jlg9pw	Silva, Camila S	Human Respiratory Coronaviruses Detected In Patients with Influenza-Like Illness in Arkansas, USA	2014-03-26		.txt	text/plain	4255	221	57	Our study aim was to investigate the molecular epidemiology of human CoV strains circulating in Arkansas, their genetic variability and their association with reported influenza-like symptoms. Samples were pre-screened, using a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) multiprobe for coronavirus, and subjected to confirmatory pancoronavirus and/or strain-specific reverse transcriptase (RT)-PCR followed by sequence analysis. RNAs positive for CoV by qRT-PCR were analyzed by one-step RT-PCR, using degenerate primers for the ORF1b [20] and specific primers (Table 1) for the spike and nucleocapsid genes of human alphacoronavirus (NL63 and 229E) and betacoronavirus (OC43 and HKU1). Among the 3 feline-like samples (based on ORF1b phylogenetic analysis), two were amplified and sequenced using primers to the S region, one was shown to share the highest identity with the OC43 strain (95.9% identity) and another shared the highest identity to feline CoV (83.8% identity) ( Figure 2 ).	./cache/cord-291958-g4jlg9pw.txt	./txt/cord-291958-g4jlg9pw.txt