id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-288327-r20zowty	Coiras, M.T.	Oligonucleotide array for simultaneous detection of respiratory viruses using a reverse‐line blot hybridization assay	2005-04-15		.txt	text/plain	5919	286	47	Biotin‐labeled PCR products obtained with two multiplex reverse transcription (RT)‐polymerase chain reaction (PCR) assays described previously, which allow for the detection of fourteen different groups of respiratory viruses, were hybridized to the oligonucleotide array. Biotin-labeled PCR products obtained with two multiplex reverse transcription (RT)-polymerase chain reaction (PCR) assays described previously, which allow for the detection of fourteen different groups of respiratory viruses, were hybridized to the oligonucleotide array. To evaluate the validity of this method for routine diagnosis was performed a comparative analysis using reference strains from a wide range of respiratory viruses, viral isolates and clinical specimens that have been analyzed by both nested PCR and RLB assays. In conclusion, a RT-PCR-RLB method has been developed for simultaneous detection and typing of a wide range of respiratory viruses, based on the hybridization of biotinylated PCR products, obtained with two multiplex RT-PCR assays described previously, to an array of oligonucleotides that are immobilized and orientated on a nylon membrane.	./cache/cord-288327-r20zowty.txt	./txt/cord-288327-r20zowty.txt