id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287843-snra23sy	Lee, Wan‐Ji	Prevalence and molecular epidemiology of human coronavirus HKU1 in patients with acute respiratory illness	2012-11-14		.txt	text/plain	2715	157	56	In this study, a Taq-Man 1 -based real-time polymerase chain reaction (PCR) method that targets the HCoV-HKU1 open reading frame (ORF) 1a and ORF 1b genes with high sensitivity and specificity was developed and evaluated. Fifty HCoV-HKU1-positive cases were detected (2.5%) out of 1,985 clinical specimens using real-time PCR assays targeting ORF 1a and ORF 1b. To circumvent these limitations, a quantitative real-time PCR was developed and targeted a study group of 1,985 throat swab specimens collected from patients with an acute respiratory illness from January 2007 to May 2008. The aim of the real-time PCR assays developed in this study was to detect both of the HCoV-HKU1 ORF 1a and ORF 1b genes. One limitation of this study was that because clinical specimens were chosen from negative cases of acute respiratory illness in the ARI-Net laboratory surveillance system [Chun et al., 2009] , dual or multiple infections of HCoV-HKU1 with other respiratory viruses could not be detected.	./cache/cord-287843-snra23sy.txt	./txt/cord-287843-snra23sy.txt