id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286555-rz88g3ze	Petrovan, Vlad	Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples	2020-07-11		.txt	text/plain	3527	156	55	The most widely used molecular method approved by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) to detect SARS-CoV-2 is the real-time reverse transcription polymerase chain reaction (qRT-PCR) [4] . The protocol for the COVID-19 PCR Diatheva Detection Kit used with Fast Gene Probe One Step Mix uses 5 µL of mix 1 mixed with 0.625 µL of mix 2, 9.375 µL of primer/probe mix, and 5 µL of RNA template, with a total volume of 20 µL. An initial interlaboratory validation was performed by the Molecular Pathology Laboratory from the University Emergency Hospital Bucharest, using the PowerCheck 2019-nCoV Real-Time PCR Kit. This study was conducted as part of a surveillance program for COVID-19 implemented by the Romanian government. To determine the analytical sensitivity of the COVID-19 commercial assays used in Romanian hospitals (PowerCheck Kogene 2019-nCoV, COVID-19 PCR Diatheva Detection Kit, and 2019-nCoV CDC EUA), we first evaluated their limit of detection (LOD) by performing 10-fold serial dilutions of the controls provided by the kits.	./cache/cord-286555-rz88g3ze.txt	./txt/cord-286555-rz88g3ze.txt