id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284423-fzz8g3rq	Wang, Hui-yu	Establishment and optimization of a liquid bead array for the simultaneous detection of ten insect-borne pathogens	2018-07-31		.txt	text/plain	4665	236	51	This study aimed to establish a liquid bead array based on Luminex xMAP technology that was able to simultaneously detect multiple insect-borne pathogens. CONCLUSIONS: This optimized liquid array detection system was high-throughput and highly specific and sensitive in screening of the insect-borne pathogens. To date, multiple molecular detection methods have been established to detect insect-borne pathogens, including reverse transcriptase-polymerase chain reaction (PCR) [8] , real-time PCR [9] , a liquid bead array [10] and a microwell membrane array [11] . In this study, we established a method that was able to simultaneously detect multiple insect-borne pathogens rapidly and effectively, including bluetongue virus (BTV), epizootic hemorrhagic disease virus of deer (EHDV), Q-fever pathogen Coxiella burnetii (CB), African swine fever virus (ASFV), West Nile fever virus (WNV), Borrelia burgdorferi (BB), vesicular stomatitis virus (VSV), Rift Valley fever virus (RVFV), Ebola virus (EBV) and Schmallenberg virus (SBV). This study established a multiplexed PCR-coupled liquid array to simultaneously detect 10 types of insect-borne pathogens.	./cache/cord-284423-fzz8g3rq.txt	./txt/cord-284423-fzz8g3rq.txt