id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282321-svoshzz8	Eboigbodin, Kevin	Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B	2016-04-11		.txt	text/plain	4409	217	48	The RT-SIBA method includes a reverse transcriptase enzyme that allows one-step reverse transcription of RNA to complementary DNA (cDNA) and simultaneous amplification and detection of the cDNA by SIBA under isothermal reaction conditions. The RT-SIBA method includes a reverse transcriptase enzyme that allows a one-step reverse transcription of RNA to cDNA and simultaneous amplification and detection of the cDNA with SIBA under isothermal reaction conditions. The use of an Influenza A assay IO with no seeding region resulted in reactions with the longest detection time, suggesting that the seeding region is of vital importance for efficient amplification of the target DNA. The recombinasemediated target duplex separation and polymerase-mediated extension are the basis for exponential amplification conclusion, IOs for both influenza A and B assays used in the following experiments were designed to contain seeding regions consisting of a polyC sequence of 10 and 14 nucleotides in length, respectively.	./cache/cord-282321-svoshzz8.txt	./txt/cord-282321-svoshzz8.txt