id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281653-zogtpm7a	Thurman, Kathleen A.	Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay()	2011-03-11		.txt	text/plain	3307	195	45	A multiplex real-time PCR assay for the detection of Mycoplasma pneumoniae (MP181), Chlamydia (Chlamydophila) pneumoniae (CP-Arg), Legionella spp. This procedure may allow for a practical and efficient means to test respiratory clinical specimens for atypical pneumonia agents in health care settings and facilitate an appropriate public health response to outbreaks. Specific real-time PCR assays have been developed to provide a more rapid and reliable method for detecting respiratory pathogens in clinical specimens (Apfalter et al., 2003; Hayden et al., 2001; Mitchell et al., 2009; Winchell et al., 2008) . The current study reports the development and evaluation of a multiplex real-time PCR assay for simultaneous detection of 3 atypical bacterial pneumonia-causing organisms in clinical specimens. The sensitivity observed when testing clinical specimens with the multiplex assay was found to be significantly greater in 2 agent-specific assays (CP-Arg and Pan-Leg) and the RNase P. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions	./cache/cord-281653-zogtpm7a.txt	./txt/cord-281653-zogtpm7a.txt