id author title date pages extension mime words sentences flesch summary cache txt cord-279980-49yv65gm WANARATANA, S. The potential of house flies to act as a vector of avian influenza subtype H5N1 under experimental conditions 2010-12-01 .txt text/plain 4317 240 58 The objective of the present study was to determine the potential for house flies (Musca domestica L.) (Diptera: Muscidae) to harbour the avian influenza (AI) H5N1 virus. Before initiation of all experiments the adult stage flies were randomly selected and confirmed to be free of the AI H5N1 virus by the real-time reverse transcription-polymerase chain reaction (RRT-PCR) assay using specific primers and probes to the influenza A matrix (M) gene. The BHI washing fluid (W1) was inoculated into six 10-day-old embryonated chicken eggs to evaluate the presence of the AI H5N1 virus on the external surface of the house flies. The W2 washing fluid was inoculated into six 10-dayold embryonated chicken eggs to confirm the absence of the AI H5N1 virus on the external surface of house flies. Avian influenza (AI) H5N1 virus titre between house fly homogenates and W1 of the AI H5N1 virus exposed house flies at different times of post-exposure in embryonated chicken eggs. ./cache/cord-279980-49yv65gm.txt ./txt/cord-279980-49yv65gm.txt