id author title date pages extension mime words sentences flesch summary cache txt cord-277838-931sco95 Erles, Kerstin Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease 2003-06-05 .txt text/plain 5077 270 60 Sequence analysis of four positive samples showed the presence of a coronavirus with high similarity to both bovine and human coronavirus (strain OC43) in their polymerase and spike genes, whereas there was a low similarity to comparable genes in the enteric canine coronavirus. This investigation sought to detect coronaviruses associated with CIRD in a large kenneled dog population with a history of endemic respiratory disease, using virus culture and PCR techniques as well as serology on paired serum samples. Of the group of dogs which developed respiratory disease, 17 were positive for antibodies to CRCV on the day of entry into the kennel and 64 were negative. Furthermore, serological analysis revealed that dogs with antibodies to CRCV on the day of entry into the kennel developed respiratory disease less frequently than dogs RT-PCR results from tracheal and lung samples of 119 dogs with different respiratory signs (none to severe) using a nested PCR directed against the coronavirus spike gene. ./cache/cord-277838-931sco95.txt ./txt/cord-277838-931sco95.txt