id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274828-67yeag50	Dybkær, Karen	Identification of acute myeloid leukemia patients with diminished expression of CD13 myeloid transcripts by competitive reverse transcription polymerase chain reaction (RT-PCR)	2000-04-21		.txt	text/plain	6048	342	50	In this study we determine surface expression and transcript levels of CD13 within mononuclear cells originating from 40 AML patients with median blast percent of 90% and four healthy controls. Mononuclear cells of bone marrow aspirations from 40 AML patients and four healthy controls were analysed by competitive RT-PCR, and content of CD13 and GAPDH transcripts were determined. For the 32 AML patients and the four healthy controls having intermediate to high values of normalised CD13 transcripts levels there was a linear correlation between normalised CD13 transcript levels and cell surface expression of CD13 (r = 0.59, P B 0.001, n = 36). Such differences of up to 200-fold variation in normalised transcript levels were found only for AML patients having less than 15% surface expression of CD13 antigen. The diversity of normalised CD13 transcript levels found for patients with less than 15% CD13 antigen on the surface of their mononuclear cells was not caused by limitations of the competitive RT-PCR method.	./cache/cord-274828-67yeag50.txt	./txt/cord-274828-67yeag50.txt