id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274805-b3mqkfhh	Onodera, Kenji	Selection for 3′ end triplets for polymerase chain reaction primers	2004-12-31		.txt	text/plain	2271	112	58	We analyzed 3′ end triplets of PCR primer sequences obtained from refereed journal articles, to test those recommendations and to make empirical recommendations for primer design. The frequencies of the 64 possible 3′end triplets among 2137 PCR primers from the VirOligo database were not uniformly distributed. The analysis results revealed preferred and disfavored 3 0 end triplets in successful primer sequences, and led to recommendations for primer design based on experience rather than theory. Since the primer sequence data set contained 134 BHV-1, 237 BVDV and 121 FMV primers, these viruses were used to test whether genome compositions determined the 3 0 end triplet frequencies. Low GCC contents in the 3 0 end triplets of PCR primers were not frequently reported in the VirOligo database. We suggest that primer design software incorporate scores based on empirical frequencies of 3 0 end triplets, such as those reported here, into the evaluation of oligonucleotides as primers.	./cache/cord-274805-b3mqkfhh.txt	./txt/cord-274805-b3mqkfhh.txt