id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274676-wtizb7hk	Lee, Seung-Hun	Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea	2016-10-15		.txt	text/plain	4374	272	56	Cryptosporidium prevalence was assessed by PCR and ELISA, and molecular characterization was performed by targeting the 18S rRNA, heat-shock protein 70 (hsp70), and glycoprotein 60 (gp60) genes. Previous studies using different methods found variable prevalence rates of Cryptosporidium in cattle: 49.4% (39/79) in Hungary using IFA (Plutzer and Karanis, 2007) , 11.9% (68/571) in the United States using PCR (Fayer et al., 2006) , 75.0% (60/80) in Japan using PCR (Karanis et al., 2010) , 20.0% (15/60) in New Zealand using IFA (Shrestha et al., 2014) , 5.1% (150/2945) in China using PCR (Zhang et al., 2015) , 21.5-22.5% in Ireland using direct fluorescence antibody testing (Mirhashemi et al., 2016) , and 10.2% (49/480) in Egypt using microscopy with staining (Ibrahim et al., 2016) . In addition, the PCR and ELISA results showed the same statistically significant differences with respect to the higher prevalence in the central region and lower prevalence in the case of hemorrhagic diarrhea. In addition, a higher prevalence at the farm level than the individual level was observed, regardless of the PCR or ELISA results, indicating a wide distribution of Cryptosporidium in calves in Korea.	./cache/cord-274676-wtizb7hk.txt	./txt/cord-274676-wtizb7hk.txt