id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270579-rf933a0y	van Kruijssen, Alida M.	Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis	2006-12-20		.txt	text/plain	513	33	55	title: Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis The use of a multiplex respiratory real-time PCR in patients clinically suspected of pertussis increases the number of pathogens detected. Pathogens were detected by PCR in 31 out of 38 (82%) cases and 10 out of 21 (48%) cases in Group I and Group II, respectively. PCR can also give falsenegative results and fewer diagnoses in those patients with pertussis could be due to the quality of the sample. Other pathogens were detected in 31 out of 38 patients with clinical diagnosis of pertussis. Other pathogens that cause a pertussis-like disease have previously been described using serological assays and Cherry et al. pertussis and other pathogens causing pertussis-like symptoms and use of this form of diagnosis might help in treatment and improve management of patients. Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis	./cache/cord-270579-rf933a0y.txt	./txt/cord-270579-rf933a0y.txt