id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265978-i0fu8e0p	Amer, Haitham M.	Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus	2011-06-30		.txt	text/plain	4797	227	49	In this report, a real-time RT-PCR system using SYBR Green I and melt curve analysis was developed for detection and quantification of BCoV in clinical samples. The reaction conditions were first optimized by testing variable concentrations of BCoV and internal control QPCR primer sets; MgCl 2 concentrations; template volumes and primer annealing Following amplification, a melt curve analysis was performed to verify the specificity of the amplified products by their specific melting temperatures (Tm). In order to determine the optimal conditions for developing a robust SYBR Green I based real-time qPCR assay that detects BCoV and internal control RNA simultaneously, different variables of the reaction were assessed. The competence of the developed real-time RT-PCR assay, for accurate detection of BCoV in clinical samples, was evaluated by analysis of 103 swab samples (68 fecal and 35 nasal) in comparison to the gel-based RT-PCR assay ( Table 4 ).	./cache/cord-265978-i0fu8e0p.txt	./txt/cord-265978-i0fu8e0p.txt