id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263417-jgu8kc5k	Yan, Yong	A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses	2017-06-17		.txt	text/plain	4657	189	45	We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). In this study, one-step multiplex reverse transcription-PCR(RT-PCR) and Luminex xMAP technology were utilized to develop a respiratory multiplex LiquiChip assay (rMLA) for the detection of 6 common respiratory viruses in Jiaxing, including influenza virus type A (FluA) and B (FluB), parainfluenza virus type 3 (PIV-3), respiratory syncytial virus (RSV) and human metapneumovirus (MPV), as well as a potentially threatening virus, MERS-CoV [29] . The analytical sensitivities of the Luminex-based rMLA and multiplex real-time RT-PCR assay developed in this study were assessed by testing in duplicate 10-fold serial dilutions of positive standards ranging from 10 6 to 10 1 copies/μl of viral RNA transcripts for each target.	./cache/cord-263417-jgu8kc5k.txt	./txt/cord-263417-jgu8kc5k.txt