id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263134-0p4zy5t2	de Paz, Hector David	Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics	2014-07-23		.txt	text/plain	6474	304	34	This review describes the landscape of molecular isothermal diagnostic techniques for infectious diseases, their characteristics, current state of development, and available products, with a focus on new directions towards point-of-care applications. Advances in microfluidics and miniaturization of signal detectors have allowed the integration of molecular diagnostics into microscale lab-on-achip devices that perform all necessary PCR steps automatically, from sample intake to cell lysis, DNA extraction, purification and amplification. This review describes the state-ofthe-art and new directions in the development of isothermal amplification technologies for diagnosis of infectious diseases with particular focus on those susceptible to be integrated in inexpensive molecular POC tests. • Loop-mediated isothermal amplification, smart amplification process & signal mediated amplification of RNA technology, helicasedependent amplification, strand displacement amplification, recombinase polymerase amplification and nicking and extension amplification reaction are isothermal techniques with mid/high tolerance to inhibitory compounds that allow the use of raw samples without any pretreatment step, which may be an interesting feature for PCR-based point-of-care (POC) testing.	./cache/cord-263134-0p4zy5t2.txt	./txt/cord-263134-0p4zy5t2.txt