id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261823-pgluidj8	Wang, Ji	Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2	2020-05-22		.txt	text/plain	3581	183	60	In this study, we have developed and evaluated a novel one-step single-tube nested quantitative real-time PCR (OSN-qRT-PCR) assay for the highly sensitive detection of SARS-CoV-2 targeting the ORF1ab and N genes. 7−10 In this study, we developed and evaluated an OSN-qRT-PCR assay for the highly sensitive detection of SARS-CoV-2, targeting the ORF1ab and N genes on the basis of a commercial qRT-PCR kit for SARS-CoV-2 (Sansure, Hunan, China). Compared to this qRT-PCR kit, the OSN-qRT-PCR assay revealed higher sensitivity and specificity, showing better suitability to clinical applications for the detection of SARS-CoV-2 in patients with low viral load. Compared to this qRT-PCR kit, the OSN-qRT-PCR assay revealed higher sensitivity and specificity, showing better suitability to clinical applications for the detection of SARS-CoV-2 in patients with low viral load. Collectively, the OSN-qRT-PCR assay has advantages of high sensitivity and easy applicability for the detection of SARS-CoV-2 in clinical samples.	./cache/cord-261823-pgluidj8.txt	./txt/cord-261823-pgluidj8.txt