id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261329-k1p7fo0e	Nidzworski, Dawid	Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR	2010-12-28		.txt	text/plain	3156	199	58	A rapid diagnostic method based on the melting curve SYBR Green I real-time PCR analysis was developed to detect and differentiate Newcastle disease virus (NDV) strains. The results obtained in this study demonstrate the possible applications for melting curve real-time PCR analysis in laboratory practice for the diagnosis and differentiation of avirulent and virulent strains of Newcastle disease virus. (2005) described a SYBR Green I real-time PCR melting curve analysis assay for differentiation, although the differences in the Tm values between the three genotypes were not very significant and could cause false characterization of the virus. Using the SYBR Green I real-time PCR melting peak analysis, it was possible to detect and differentiate virulent and avirulent strains of Newcastle disease virus. In this study, a method for the rapid detection and differentiation of Newcastle disease virus by SYBR Green I melting-curve analysis was described.	./cache/cord-261329-k1p7fo0e.txt	./txt/cord-261329-k1p7fo0e.txt