id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261237-0hbijukt	Hou, Peili	Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus	2017-12-26		.txt	text/plain	4858	254	51	title: Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. In this study, we aimed to develop the lateral flow dipsticks recombinase polymerase amplification (LFD-RPA) assays for rapid detection of BEFV. The results of those assays showed that a total of 83 clinical specimens were tested positive by conventional RT-PCR, while the similar performance that 96 specimens were detected positive by BEFV RPA nucleic acid amplification assays on LFD within 5 min, and 95 specimens were positive with the C t values below 35 using the real-time qPCR assay. As the applications of LFD-RPA, conventional RT-PCR and real time qPCR methods for detection BEFV genomes from clinical samples (Table 2) , the results clearly indicated the potential benefits of the developed assay over PCR-based methods.	./cache/cord-261237-0hbijukt.txt	./txt/cord-261237-0hbijukt.txt