id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261134-zarq507s	Pulford, David	Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus	2004-02-13		.txt	text/plain	3799	215	50	PCR assays that can identify the presence of variola virus (VARV) sequences in an unknown DNA sample were developed using principles established for the amplification refractory mutation system (ARMS). When a variola virus specific primer was used with a consensus primer in an ARMS assay with different Orthopoxvirus genomes, a PCR product was only amplified from variola virus DNA. Incorporating a second consensus primer into the assay produced a multiplex PCR that provided Orthopoxvirus generic and variola-specific products with variola virus DNA. The variola virus specific primers did not produce amplicons with either assay format when tested with 50 other Orthopoxvirus DNA samples. These multiplex assays employ three primers; two consensus primers generate an amplicon diagnostic of an Old World Orthopoxvirus and the third primer simultaneously binds to a variola-specific polymorphism and initiates extension of a shorter PCR product to detect the presence of variola virus.	./cache/cord-261134-zarq507s.txt	./txt/cord-261134-zarq507s.txt