id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259988-3s7b5ovi	Decaro, Nicola	Virological and molecular characterization of a mammalian orthoreovirus type 3 strain isolated from a dog in Italy	2005-08-10		.txt	text/plain	3336	191	57	A mammalian orthoreovirus (MRV) strain was isolated from a pup with fatal diarrhea, which had a concurrent infection by canine parvovirus type 2. Assignment of the isolated virus to MRV-3 was confirmed by type-specific RT-PCR assays, targeting the S1 gene, and by subsequent sequence analysis of the PCR product. A total of 110 fecal samples, 56 nasal and 31 ocular swabs from dogs with diarrhea or nasal/ocular discharge were tested by a nested-PCR assay specific for reoviruses, and no sample was found to contain MRV RNA, a finding that is apparently in contrast with the seroprevalence (25.77%) observed in dogs. In the present study, the isolation and molecular characterization of a MRV-3 strain from a dog with diarrhea are reported. By the type-specific RT-PCR assays the isolate was recognized as MRV-3 (Fig. 5) , and therefore designated T3/ canine/Italy/Decaro/2004 (T3D/04), according to the conventional system used to identify MRV strains.	./cache/cord-259988-3s7b5ovi.txt	./txt/cord-259988-3s7b5ovi.txt