id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259886-j0bpp7iw	Cho, Hyejin	Positive control synthesis method for COVID-19 diagnosis by one-step real-time RT-PCR	2020-10-12		.txt	text/plain	2319	136	53	SPT oligonucleotides contain probe binding and virus-irrelevant regions as templates for detecting SARS-CoV-2 genes (RdRP, E, and N SARS-CoV-2) by real-time RT-PCR was performed in a concentration-dependent manner. Therefore, this approach may be integrated into the molecular diagnosis of COVID-19 and provides a general method for preparing positive controls for diagnosing emerging RNA virus infections. Therefore, a new preparation design that provides contamination-free positive controls for COVID-19 real-time RT-PCR testing is urgently needed. Here, we present a new approach for producing synthetic positive controls using synthetic positive template (SPT) oligonucleotides that contain probe binding and virusirrelevant regions as templates for detecting SARS-CoV-2 genes by real-time RT-PCR. We performed multiplex real-time RT-PCR using an SPT (positive control) as a template in a concentration-dependent manner, to determine the limit of detection (LOD) for individual SARS-CoV-2 genes. In previous studies, the Uni-Control method for preparing real-time RT-PCR positive controls has been developed as a generic approach for diagnosing viral infections [22] .	./cache/cord-259886-j0bpp7iw.txt	./txt/cord-259886-j0bpp7iw.txt