id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259324-g8kv4pvq	Ko, Suk-Min	Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor	2011-10-28		.txt	text/plain	2332	121	50	In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. In this study, we report the stable transformation and expression of a protective antigen for PEDV in Lemna minor with potential for use as an effective complement to the diets of animals. Fronds were then blotted onto sterile filter paper, and co-cultivated with Agrobacterium tumefaciens strain EHA105 harboring the PEDV spike protein 1 gene fused to a c-myc tag for 72 h on antibiotic-free ½MS1BA medium. PCR products of the expected size (330 bp) corresponding to primers designed on the internal PEDV spike protein 1 gene were detected from kanamycin-resistant Lemna, whereas no DNA band corresponding to the target gene was detected in untransformed wild-type Lemna.	./cache/cord-259324-g8kv4pvq.txt	./txt/cord-259324-g8kv4pvq.txt