id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257284-dash9udv	Decaro, Nicola	Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1	2010-07-30		.txt	text/plain	3138	149	52	The detection limit was 10(1) and 1.20 × 10(1) DNA copies per 10 μl(−1) of template for standard DNA and a CHV-1-positive kidney sample, respectively: about 1-log higher than a gel-based PCR assay targeting the thymidine kinase gene. Unlike other CHV-1-specific diagnostic methods, this quantitative assay permits simultaneous detection and quantitation of CHV-1 DNA in a wide range of canine tissues and body fluids, thus providing a useful tool for confirmation of a clinical diagnosis, for the study of viral pathogenesis and for evaluation of the efficacy of vaccines and antiviral drugs. To evaluate the detection limits of the real-time PCR assay, 10fold dilutions of the plasmid DNA, ranging from 10 9 to 10 0 copies, were made in a CHV-1-negative kidney homogenate and tested subsequently. The development and validation of a real-time PCR assay for detection and absolute quantitation of CHV-1 DNA in tissue samples and body fluids of dogs are described.	./cache/cord-257284-dash9udv.txt	./txt/cord-257284-dash9udv.txt