id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255545-nycdhdsd	Schoenike, Barry	Quantitative sense-specific determination of murine coronavirus RNA by reverse transcription polymerase chain reaction	1999-03-10		.txt	text/plain	6170	265	48	In theory, sense-specific measurement of viral RNAs may be achieved by reverse transcription polymerase chain reaction (RT-PCR) assays which utilize primers of defined polarity during the RT step. Key RT-PCR parameters which were optimized include the design of tagged primers, DNase treatment of in vitro transcribed RNA standards, specification of temperature differences between RT and PCR annealing steps, and use of competitive RNA templates for quantitative assays. In fact, several studies have demonstrated that RT-PCR assays based on specific RNA template recognition by RT primers of defined polarity will not reliably distinguish between viral RNAs of positive or negative sense. Despite the findings above, many published research reports are based on conventional RT-PCR assays, relying on the polarity of primers added to the RT step in putative sense-specific measurements of viral RNAs; rarely are control reactions performed to rigorously show that this method is in fact sense-specific.	./cache/cord-255545-nycdhdsd.txt	./txt/cord-255545-nycdhdsd.txt