id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102511-7zgd45fl	Khodakov, Dmitriy	Donut PCR: a rapid, portable, multiplexed, and quantitative DNA detection platform with single-nucleotide specificity	2020-05-05		.txt	text/plain	4279	214	44	Here, we present the Donut PCR platform that features high multiplexing, rapid turnaround times, single nucleotide discrimination, and precise quantitation of DNA targets in a portable, affordable, and battery-powered instrument using closed consumables that minimize contamination. Here, we present the Donut PCR platform for DNA detection that combines scalable and massive multiplexing, rapid turnaround times, single nucleotide discrimination, and precise quantitation in a portable, affordable, and batterypowered instrument using closed consumables that minimize contamination risks ( Table 1 ). By engineering a donut-shaped reaction chamber in the PCR chip, we remove most of the dead volume, and are able to achieve similar PCR specificity on human genomic DNA as the commercial Bio-Rad CFX96 instrument (Fig. 2a) . The Donut PCR platform presented here achieves rapid, sensitive, and quantitative detection of many DNA targets from a single sample using a closed, portable, and affordable instrument.	./cache/cord-102511-7zgd45fl.txt	./txt/cord-102511-7zgd45fl.txt