id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048470-33mqfj2t	Takano, T	Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy	2001-07-17		.txt	text/plain	3040	146	54	Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Recent reports have demonstrated that the reverse transcription-polymerase chain reaction (RT-PCR) can be used to detect circulating cancer cells in the peripheral blood of patients with malignancies such as prostate cancer (Ghossein et al, 1995) and neuroblastoma (Mattano et al, 1992) . As such, we measured TG mRNA in the peripheral blood of patients after total thyroidectomy and determined the expression levels of TG mRNA by real time-quantitative RT-PCR using GAPDH mRNA as an internal control.	./cache/cord-048470-33mqfj2t.txt	./txt/cord-048470-33mqfj2t.txt