id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048359-lz37rh82	Li, Jin	s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis	2007-06-01		.txt	text/plain	6258	299	49	Subsequently, melting curve analysis, on conventional or nano-technology real-time PCR platforms, detects the samples that contain mutations in a high-throughput and closed-tube manner. Following denaturation and re-annealing of PCR products that leads to formation of cross-hybridized sequences at the positions of mutations ( Figure 1A ) the sample is exposed to Surveyor TM endonuclease that recognizes base pair mismatches or small loops with high specificity (28) and generates a break on both DNA strands 3 0 to the mismatch. Finally, because the amplified mutated sequences contain defined primers at their ends, direct sequencing of enzymatically selected PCR products is readily possible following the real-time melting step, enabling sequencing of low-level mutations identified by Surveyor TM . Here we enabled Surveyor TM , an endonuclease that recognizes selectively mismatches formed by mutations and small deletions following 'cross-hybridized sequence' formation, to generate mutation-specific DNA fragments that are amplified and screened via differential melting curve analysis.	./cache/cord-048359-lz37rh82.txt	./txt/cord-048359-lz37rh82.txt