id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-023830-w218ogsk	Perlin, David	Rapid Detection of Bioterrorism Pathogens	2008-09-10		.txt	text/plain	6048	292	38	The inadequacy of phenotypic-based diagnostic assays is illustrated graphically by the ''gold standard'' public health laboratory-testing algorithm that was in place for positive identification of Bacillus anthracis from environmental samples during the October 2001 anthrax outbreak (Fig. 16.1a) . Genomic differences between microbes offer an alternative to culturing for detection and identification of pathogens by providing species-specific DNA targets that can be accurately resolved by molecular methodology. Polymerase chain reaction (PCR)-based amplification of highly conserved ribosomal RNA (rRNA) genes, intergenic sequences, and specific toxin genes is currently the most reliable approach for identification of bacterial, fungal and many viral pathogenic agents. Most importantly, these genetic probing systems offer rapid turn around time (1-6 h) and are suitable for high throughput, automated multiplex operations critical for use in clinical diagnostic laboratories. Rapid diagnostic assays in the genomic biology era: detection and identification of infectious disease and biological weapon agents	./cache/cord-023830-w218ogsk.txt	./txt/cord-023830-w218ogsk.txt