id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-018865-melttpiq	Yu, Tian-fei	Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems	2012		.txt	text/plain	2186	126	53	In order to illuminate the antigenicity of porcine transmissible gastroenteritis virus (TGEV) spike protein B and C antigen sites, the truncated spike gene including B and C antigen sites of Chinese isolate TH-98 was expressed respectively in E.coli, baculovirus and pichia pastoris expression systems. Dot-ELISA assays based on these three recombinant proteins were developed to detect TGEV antibodies and could avoid antibody cross-reaction from PRCV theoretically. The recombinant B and C antigen sites protein expressed into the yeast culture supernatant was identified on the bases of its molecular weight. When the amount of spotting is 50ng, the recombinant B and C antigen sites protein expressed into the yeast culture supernatant show the positive reaction in contrast with the GS115 cells transformed with pPIC9K plasmids (Fig. 2. Antigenic variation among transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the S protein of TGEV	./cache/cord-018865-melttpiq.txt	./txt/cord-018865-melttpiq.txt