id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-017363-dmb42kna	Vemulapalli, Ramesh	Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus	2015-09-10		.txt	text/plain	1069	86	63	Transmissible gastroenteritis (TGE) is a highly contagious disease of pigs caused by the TGE virus (TGEV). Rapid detection of the virus in the affected pigs' feces is critical for controlling the disease outbreaks. The real-time RT-PCR assay described in this chapter can quickly detect the presence of TGEV in fecal samples with high sensitivity and specificity. The assay, along with the RNA extraction method described here, can be established in any molecular diagnostic laboratory for detection of TGEV in pig fecal samples [ 6 -8 ] . It is recommended that the nucleic acid extraction, preparation of master mix, and real-time PCR amplifi cation are performed in three physically separated areas. In our experience, RNA extracted from pig fecal samples using the following method is suitable for TGEV detection using the real-time RT-PCR assay. A real-time TaqMan ® RT-PCR assay with an internal amplifi cation control for rapid detection of transmissible gastroenteritis virus in swine fecal samples	./cache/cord-017363-dmb42kna.txt	./txt/cord-017363-dmb42kna.txt