id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016417-3cwwmyv9	Sluijter, J. P. G.	Quantitative Real-Time PCR	2006		.txt	text/plain	3110	174	53	In this chapter, we focus on the newest advancements in reverse transcription polymerase chain reaction (rt-pcr) technology, the real-time PCR or quantitative PCR, using small amounts of RNA to determine expression levels.We discuss the technique in general and describe two different approaches. Traditional methods to quantify mRNA expression levels are Northern blotting, in situ hybridization, ribonuclease protection, cDNA arrays, and reverse transcription polymerase chain reaction (rt-pcr). PCR amplification of your target DNA will increase the number of probes that hybridize with the complementary template. Accumulation of the released reporter molecules during the amplification cycles results in an increasing fluorescent signal and is correlated to the amount of the target DNA present. When the probe binds to the target sequence this will result in a separation of the reporter and quencher molecule and the fluorescent signal can be monitored. Amplification in a real-time PCR will increase the target DNA and therefore also the observed fluorescent signal.	./cache/cord-016417-3cwwmyv9.txt	./txt/cord-016417-3cwwmyv9.txt