id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007427-iqwojhq2	Dedkov, Vladimir G.	Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus	2019-06-03		.txt	text/plain	4043	213	53	Based on sequencing data, LASV-specific assay was developed using synthetic MS2-phage-based armored RNA particles, RNA from Lassa virus strain Josiah, and further, evaluated in field conditions using samples from patients and Mastomys natalensis rodents. Viral RNAs were examined for Lassa immediately after extraction by the staff of the Virology Laboratory of Hemorrhagic Fevers Research Project of Gamal Abdel Nasser University of Conakry, Guinea and were then used to assess diagnostic sensitivity and specificity. In addition, LOD was assessed using a series of 10-fold dilutions of ARPs. For this purpose, eight LASV sequences of a maximal number of mismatches in the targeting region of L gene were selected (including a sequence of the strain Josiah, which was also used for the generation of the positive controls) and generated for the production of ARPs as described above (Table 2 ) .	./cache/cord-007427-iqwojhq2.txt	./txt/cord-007427-iqwojhq2.txt