id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004003-rlgzgyzn	Lee, Jeewon	Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification	2019-11-12		.txt	text/plain	3386	200	51	Thus, to amplify the size-variable DNA library uniformly, we introduced a linear amplification strategy with RPA and successfully improved the uniformity. Also, the average percentages of bases in the uniform range (uniformity value between 0.5 and 1.5) about the triplet experiment was improved from 56.3 and 49.2% by two-primer RPA to 73.6 and 75.7% by linear RPA for the small and large DNA libraries, respectively (Figures 2b and S5) . Taken together, the data show that RPA uniformly amplifies DNA libraries of the same size and has different amplification preferences than PCR. However, during the experiment, an accelerated small-sized DNA amplification by two-primer RPA reaction caused lower uniformity compared to PCR. It was noted that during the analysis, different amplification preferences were found between the PCR and RPA amplified oligo library sequencing data. Taken together, we show that single-primer linear RPA can be one of the alternative methods to PCR for DNA library amplification.	./cache/cord-004003-rlgzgyzn.txt	./txt/cord-004003-rlgzgyzn.txt