id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003505-qr6ukfti	Tabraue-Chávez, Mavys	A colorimetric strategy based on dynamic chemistry for direct detection of Trypanosomatid species	2019-03-06		.txt	text/plain	5655	331	50	In this work, we have developed a novel colorimetric molecular assay that integrates nucleic acid analysis by dynamic chemistry (ChemNAT) with reverse dot-blot hybridization in an array format for a rapid and easy discrimination of Leishmania major and Trypanosoma cruzi. Once the abasic PNA probes hybridize with target sequences, the SMART-C-Biotin dynamic incorporation takes place, enabling the unequivocal identification of the parasite present in the amplicon sample, because of the unique colorimetric pattern that each Trypanosomatid amplicon generates. DNA amplicon products were denatured and then together with the dynamic chemistry reaction reagents added directly into the internal column of the Spin-Tube that supports the nylon membrane for the color-development assay (Fig. 4) . 20 ng of human gDNA were used as PCR template for its amplification with the set of primers described in our study and neither colorimetric signals nor bands were detected using the Spin-Tube and capillary electrophoresis analysis respectively (Fig. 5 , column 2: 2A and 2B), hence probing the specificity when human gDNA is present.	./cache/cord-003505-qr6ukfti.txt	./txt/cord-003505-qr6ukfti.txt